exosome markers cd63  (Proteintech)

Journal: Stem Cell Research & Therapy

Article Title: Protective effects of mouse bone marrow mesenchymal stem cell-derived extracellular vesicles on radiation-induced epididymal cells damage via USP44/RBM14 axis

doi: 10.1186/s13287-025-04782-9

Figure Lengend Snippet: Characterization of mBMSC and Their EVs. A Flow cytometry histograms showing expression of surface markers. Cells were negative for hematopoietic markers CD14, CD19, CD34, and CD45 (< 0.5%) and positive for mesenchymal markers CD29, CD44, CD73, and CD90 (> 98%). Gray shading represents isotype controls; red lines represent specific antibody staining. B Representative images of lineage-specific staining after differentiation induction: Alizarin Red (osteogenesis), Alcian Blue (chondrogenesis), and Oil Red O (adipogenesis). Scale bars, 100 μm. C Transmission electron microscopy images of EVs showing typical morphology and size. Scale bars represent 0.5 μm (left) and 200 nm (right). D Nanoparticle tracking analysis illustrating extracellular vesicle size distribution and concentration, with a peak around 100 nm. E Western blot detection of typical exosomal markers CD9, CD63, and CD81, confirming vesicle identity

Article Snippet: Exosomal protein markers CD9 (Proteintech, #20,597–1-AP, 1:2000), CD81 (Proteintech, #27,855–1-AP, 1:2000) and CD63 (Proteintech, #67,605–1-Ig, 1:5000) were detected by Western blotting.

Techniques: Flow Cytometry, Expressing, Staining, Transmission Assay, Electron Microscopy, Concentration Assay, Western Blot

Journal: Molecular Neurobiology

Article Title: The Combination of Sodium Tanshinone IIA Sulfonate and Mesenchymal Stem Cell-derived Exosomes Against Spinal Cord Injury via VEGF Pathway

doi: 10.1007/s12035-025-05422-2

Figure Lengend Snippet: Identification of BMSCs-Exo and enhancement of bEnd.3 cells vitality after H 2 O 2 damage by STS combined with Exo. A The morphology of extracellular vesicles was observed by transmission electron microscopy. B-C Size distribution of extracted extracellular vesicles. D Western blot showed that the expression of exosome marker proteins CD63 and TSG101 was detected in extracellular vesicles. E The structure of STS. F The bEnd.3 cells were treated with different concentrations (1, 3, and 10 µM) of STS for 24 h. G The bEnd.3 cells were treated with 300 µM H 2 O 2 for 0, 12, 24, and 48 h. Cell viability was assayed by cck-8. H Treatment of bEnd.3 cells with different concentrations (100, 200, 300, 400, and 500 µM) of H 2 O 2 for 24 h. I Effect of different concentrations of STS (1, 3, and 10 µM) on cell viability in H 2 O 2 -treated bEnd.3 cells. J 300 µM H 2 O 2 was selected to induce cellular oxidative stress, and cell survival was detected using 3 µM STS and 10 µg/ml exosomes in grouped interventions on bEnd.3 cells. Data are presented as the mean ± SD of five independent experiments. ∗ P < 0.05

Article Snippet: Before assessment of exosomal marker proteins CD63 (1:200,25,682–1-AP, Proteintech, USA), and TSG101(1:2000, ET1701-59, HUABIO, China) through Western blot (WB) assay [ ],a BCA protein assay kit (P0010, Beyotime, China) was used to quantify the protein concentration and control the amount of exosomes loaded to 10 μg.

Techniques: Transmission Assay, Electron Microscopy, Western Blot, Expressing, Marker, CCK-8 Assay